The protocol describes a method for capture of secreted hexahistidine-tagged proteins using expanded-bed adsorption immobilized-metal affinity chromatography. The starting material for the procedure ...

Synthetic dyes can act as alternative ligands for proteins and can be used as part of a protein purification protocol for chromatography. Chromatography is the separation of compounds from a mixture.

The present purification protocol applies to target proteins that are fused to a double tag, such as NusA–His 6, through a linker that includes a protease-recognition sequence. It involves two steps ...

Antibodies are essential proteins produced by the immune system that recognize and bind to specific targets, known as antigens. Their high specificity and affinity make antibodies invaluable tools in ...