Although efficiency of adenovirus vector creation by homologous recombination in 293 cells was improved by a green/white selection process, 17 the initial isolates still consist of mixtures of ...

Young scientists often get frustrated with laborious experimental procedures, such as cloning, PCR, or sequencing. However, the Internet has made planning and performing these procedures faster and ...

While the traditional cloning workflow has been used in laboratories for decades, new techniques have emerged that allow for rapid assembly of multiple genetic fragments. The Electra Vector System, ...

One of the most indispensable techniques in all areas of the life sciences is the use of expression vectors. Cloning a gene between two restriction sites, and the expression of a protein, are almost ...

As a lab within the Cooperative Center of Excellence in Hematology (CCEH) at Fred Hutch, the Vector Production core assists investigators with the design, construction, and/or production of viral ...

Key to Invitrogen's Gateway technology is the "entry clone," which contains a fragment of interest in a recombinase-ready plasmid. Researchers can quickly and efficiently move between expression ...

Molecular cloning is a technique used to create DNA experimental tools that are expressed in either bacterial or mammalian cells. Molecular cloning is defined as the isolation and amplification of a ...