Proinsulin was expressed by Escherichia coli SHuffle T7 with pET system in minimal medium containing glucose (Glu medium), glucose and glycerol (GluGly medium) or glycerol (Gly medium). With 100 μM ...
Addressing this problem, Cytos Biotechnology AG of Zürich-Schlieren, Switzerland, has designed an inducible expression vector that uncouples transcription from induction. The system uses the vector ...
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