One-step RT-PCR just got easier 😎 👋 Say goodbye to optimizing annealing temperatures for different targets! With our new one-step RT-PCR kit, you can get accurate results in just a few steps. Learn more about our new one-step RT-PCR kit at http://spr.ly/6187Pu95c #RTPCR #molecularbiology #highthroughput | Thermo Scientific Biology
One-step RT-PCR just got easier 😎 👋 Say goodbye to optimizing anneali…
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Benefits of a universal annealing temperature for PCR - Scientific Videos | Thermo Fisher Scientific US
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Primer annealing is the key to PCR success. To maximize yield and specificity, the annealing temperature of each primer set should be optimized. Optimizing annealing temperatures can be time consuming and tedious, particularly when amplifying multiple DNA targets using different primer sets. Hear from our scientists about the innovative reaction buffers that allow a universal annealing temperature of 60°C! Explore more about the Invitrogen Platinum DNA polymerases with universal annealing buffer
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SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 0.41 (%GC) - (675/N), where %GC is the percentage of G and C nucleotides in the oligonucleotide and N is the length of the oligonucleotide. Notice from the formula that both the GC content and the length of the oligonucleotide are variables. Assuming you have the following oligonucleotide as a primer, compute the annealing temp
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